Cucumber hybrid SV8975CB and parents thereof

ABSTRACT

The invention provides seed and plants of cucumber hybrid SV8975CB and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of cucumber hybrid SV8975CB and the parent lines thereof, and to methods for producing a cucumber plant produced by crossing such plants with themselves or with another cucumber plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims the priority of U.S. Provisional Appl. Ser. No.61/817,786, filed Apr. 30, 2013, the entire disclosure of which isincorporated herein by reference.

FIELD OF THE INVENTION

The present invention relates to the field of plant breeding and, morespecifically, to the development of cucumber hybrid SV8975CB and theinbred cucumber lines ISAAC and BAP-TA-MATHYS-GY.

BACKGROUND OF THE INVENTION

The goal of vegetable breeding is to combine various desirable traits ina single variety/hybrid. Such desirable traits may include any traitdeemed beneficial by a grower and/or consumer, including greater yield,resistance to insects or disease, tolerance to environmental stress, andnutritional value.

Breeding techniques take advantage of a plant's method of pollination.There are two general methods of pollination: a plant self-pollinates ifpollen from one flower is transferred to the same or another flower ofthe same plant or plant variety. A plant cross-pollinates if pollencomes to it from a flower of a different plant variety.

Plants that have been self-pollinated and selected for type over manygenerations become homozygous at almost all gene loci and produce auniform population of true breeding progeny, a homozygous plant. A crossbetween two such homozygous plants of different genotypes produces auniform population of hybrid plants that are heterozygous for many geneloci. Conversely, a cross of two plants each heterozygous at a number ofloci produces a population of hybrid plants that differ genetically andare not uniform. The resulting non-uniformity makes performanceunpredictable.

The development of uniform varieties requires the development ofhomozygous inbred plants, the crossing of these inbred plants, and theevaluation of the crosses. Pedigree breeding and recurrent selection areexamples of breeding methods that have been used to develop inbredplants from breeding populations. Those breeding methods combine thegenetic backgrounds from two or more plants or various other broad-basedsources into breeding pools from which new lines and hybrids derivedtherefrom are developed by selfing and selection of desired phenotypes.The new lines and hybrids are evaluated to determine which of those havecommercial potential.

SUMMARY OF THE INVENTION

In one aspect, the present invention provides a cucumber plant of thehybrid designated SV8975CB, the cucumber line ISAAC or cucumberBAP-TA-MATHYS-GY. Also provided are cucumber plants having all thephysiological and morphological characteristics of such a plant. Partsof these cucumber plants are also provided, for example, includingpollen, an ovule, scion, a rootstock, a fruit, and a cell of the plant.

In another aspect of the invention, a plant of cucumber hybrid SV8975CBand/or cucumber lines ISAAC and BAP-TA-MATHYS-GY comprising an addedheritable trait is provided. The heritable trait may comprise a geneticlocus that is, for example, a dominant or recessive allele. In oneembodiment of the invention, a plant of cucumber hybrid SV8975CB and/orcucumber lines ISAAC and BAP-TA-MATHYS-GY is defined as comprising asingle locus conversion. In specific embodiments of the invention, anadded genetic locus confers one or more traits such as, for example,herbicide tolerance, insect resistance, disease resistance, and modifiedcarbohydrate metabolism. In further embodiments, the trait may beconferred by a naturally occurring gene introduced into the genome of aline by backcrossing, a natural or induced mutation, or a transgeneintroduced through genetic transformation techniques into the plant or aprogenitor of any previous generation thereof. When introduced throughtransformation, a genetic locus may comprise one or more genesintegrated at a single chromosomal location.

The invention also concerns the seed of cucumber hybrid SV8975CB and/orcucumber lines ISAAC and BAP-TA-MATHYS-GY. The cucumber seed of theinvention may be provided as an essentially homogeneous population ofcucumber seed of cucumber hybrid SV8975CB and/or cucumber lines ISAACand BAP-TA-MATHYS-GY. Essentially homogeneous populations of seed aregenerally free from substantial numbers of other seed. Therefore, seedof hybrid SV8975CB and/or cucumber lines ISAAC and BAP-TA-MATHYS-GY maybe defined as forming at least about 97% of the total seed, including atleast about 98%, 99% or more of the seed. The seed population may beseparately grown to provide an essentially homogeneous population ofcucumber plants designated SV8975CB and/or cucumber lines ISAAC andBAP-TA-MATHYS-GY.

In yet another aspect of the invention, a tissue culture of regenerablecells of a cucumber plant of hybrid SV8975CB and/or cucumber lines ISAACand BAP-TA-MATHYS-GY is provided. The tissue culture will preferably becapable of regenerating cucumber plants capable of expressing all of thephysiological and morphological characteristics of the starting plant,and of regenerating plants having substantially the same genotype as thestarting plant. Examples of some of the physiological and morphologicalcharacteristics of the hybrid SV8975CB and/or cucumber lines ISAAC andBAP-TA-MATHYS-GY include those traits set forth in the tables herein.The regenerable cells in such tissue cultures may be derived, forexample, from embryos, meristems, cotyledons, pollen, leaves, anthers,roots, root tips, pistils, flowers, seed and stalks. Still further, thepresent invention provides cucumber plants regenerated from a tissueculture of the invention, the plants having all the physiological andmorphological characteristics of hybrid SV8975CB and/or cucumber linesISAAC and BAP-TA-MATHYS-GY.

In still yet another aspect of the invention, processes are provided forproducing cucumber seeds, plants and fruit, which processes generallycomprise crossing a first parent cucumber plant with a second parentcucumber plant, wherein at least one of the first or second parentcucumber plants is a plant of cucumber line ISAAC or cucumberBAP-TA-MATHYS-GY. These processes may be further exemplified asprocesses for preparing hybrid cucumber seed or plants, wherein a firstcucumber plant is crossed with a second cucumber plant of a different,distinct genotype to provide a hybrid that has, as one of its parents, aplant of cucumber line ISAAC or cucumber BAP-TA-MATHYS-GY. In theseprocesses, crossing will result in the production of seed. The seedproduction occurs regardless of whether the seed is collected or not.

In one embodiment of the invention, the first step in “crossing”comprises planting seeds of a first and second parent cucumber plant,often in proximity so that pollination will occur for example, mediatedby insect vectors. Alternatively, pollen can be transferred manually.Where the plant is self-pollinated, pollination may occur without theneed for direct human intervention other than plant cultivation.

A second step may comprise cultivating or growing the seeds of first andsecond parent cucumber plants into plants that bear flowers. A thirdstep may comprise preventing self-pollination of the plants, such as byemasculating the flowers (i.e., killing or removing the pollen).

A fourth step for a hybrid cross may comprise cross-pollination betweenthe first and second parent cucumber plants. Yet another step comprisesharvesting the seeds from at least one of the parent cucumber plants.The harvested seed can be grown to produce a cucumber plant or hybridcucumber plant.

The present invention also provides the cucumber seeds and plantsproduced by a process that comprises crossing a first parent cucumberplant with a second parent cucumber plant, wherein at least one of thefirst or second parent cucumber plants is a plant of cucumber hybridSV8975CB and/or cucumber lines ISAAC and BAP-TA-MATHYS-GY. In oneembodiment of the invention, cucumber seed and plants produced by theprocess are first generation (F₁) hybrid cucumber seed and plantsproduced by crossing a plant in accordance with the invention withanother, distinct plant. The present invention further contemplatesplant parts of such an F₁ hybrid cucumber plant, and methods of usethereof. Therefore, certain exemplary embodiments of the inventionprovide an F₁ hybrid cucumber plant and seed thereof.

In still yet another aspect, the present invention provides a method ofproducing a plant derived from hybrid SV8975CB and/or cucumber linesISAAC and BAP-TA-MATHYS-GY, the method comprising the steps of: (a)preparing a progeny plant derived from hybrid SV8975CB and/or cucumberlines ISAAC and BAP-TA-MATHYS-GY, wherein said preparing comprisescrossing a plant of the hybrid SV8975CB and/or cucumber lines ISAAC andBAP-TA-MATHYS-GY with a second plant; and (b) crossing the progeny plantwith itself or a second plant to produce a seed of a progeny plant of asubsequent generation. In further embodiments, the method mayadditionally comprise: (c) growing a progeny plant of a subsequentgeneration from said seed of a progeny plant of a subsequent generationand crossing the progeny plant of a subsequent generation with itself ora second plant; and repeating the steps for an additional 3-10generations to produce a plant derived from hybrid SV8975CB and/orcucumber lines ISAAC and BAP-TA-MATHYS-GY. The plant derived from hybridSV8975CB and/or cucumber lines ISAAC and BAP-TA-MATHYS-GY may be aninbred line, and the aforementioned repeated crossing steps may bedefined as comprising sufficient inbreeding to produce the inbred line.In the method, it may be desirable to select particular plants resultingfrom step (c) for continued crossing according to steps (b) and (c). Byselecting plants having one or more desirable traits, a plant derivedfrom hybrid SV8975CB and/or cucumber lines ISAAC and BAP-TA-MATHYS-GY isobtained which possesses some of the desirable traits of the line/hybridas well as potentially other selected traits.

In certain embodiments, the present invention provides a method ofproducing food or feed comprising: (a) obtaining a plant of cucumberhybrid SV8975CB and/or cucumber lines ISAAC and BAP-TA-MATHYS-GY,wherein the plant has been cultivated to maturity, and (b) collecting atleast one cucumber from the plant.

In still yet another aspect of the invention, the genetic complement ofcucumber hybrid SV8975CB and/or cucumber lines ISAAC andBAP-TA-MATHYS-GY is provided. The phrase “genetic complement” is used torefer to the aggregate of nucleotide sequences, the expression of whichsequences defines the phenotype of, in the present case, a cucumberplant, or a cell or tissue of that plant. A genetic complement thusrepresents the genetic makeup of a cell, tissue or plant, and a hybridgenetic complement represents the genetic make up of a hybrid cell,tissue or plant. The invention thus provides cucumber plant cells thathave a genetic complement in accordance with the cucumber plant cellsdisclosed herein, and seeds and plants containing such cells.

Plant genetic complements may be assessed by genetic marker profiles,and by the expression of phenotypic traits that are characteristic ofthe expression of the genetic complement, e.g., isozyme typing profiles.It is understood that hybrid SV8975CB and/or cucumber lines ISAAC andBAP-TA-MATHYS-GY could be identified by any of the many well knowntechniques such as, for example, Simple Sequence Length Polymorphisms(SSLPs) (Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990),Randomly Amplified Polymorphic DNAs (RAPDs), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Arbitrary Primed Polymerase Chain Reaction (AP-PCR), Amplified FragmentLength Polymorphisms (AFLPs) (EP 534 858, specifically incorporatedherein by reference in its entirety), and Single NucleotidePolymorphisms (SNPs) (Wang et al., Science, 280:1077-1082, 1998).

In still yet another aspect, the present invention provides hybridgenetic complements, as represented by cucumber plant cells, tissues,plants, and seeds, formed by the combination of a haploid geneticcomplement of a cucumber plant of the invention with a haploid geneticcomplement of a second cucumber plant, preferably, another, distinctcucumber plant. In another aspect, the present invention provides acucumber plant regenerated from a tissue culture that comprises a hybridgenetic complement of this invention.

Any embodiment discussed herein with respect to one aspect of theinvention applies to other aspects of the invention as well, unlessspecifically noted.

The term “about” is used to indicate that a value includes the standarddeviation of the mean for the device or method being employed todetermine the value. The use of the term “or” in the claims is used tomean “and/or” unless explicitly indicated to refer to alternatives onlyor the alternatives are mutually exclusive. When used in conjunctionwith the word “comprising” or other open language in the claims, thewords “a” and “an” denote “one or more,” unless specifically notedotherwise. The terms “comprise,” “have” and “include” are open-endedlinking verbs. Any forms or tenses of one or more of these verbs, suchas “comprises,” “comprising,” “has,” “having,” “includes” and“including,” are also open-ended. For example, any method that“comprises,” “has” or “includes” one or more steps is not limited topossessing only those one or more steps and also covers other unlistedsteps. Similarly, any plant that “comprises,” “has” or “includes” one ormore traits is not limited to possessing only those one or more traitsand covers other unlisted traits.

Other objects, features and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and any specificexamples provided, while indicating specific embodiments of theinvention, are given by way of illustration only, since various changesand modifications within the spirit and scope of the invention willbecome apparent to those skilled in the art from this detaileddescription.

DETAILED DESCRIPTION OF THE INVENTION

The invention provides methods and compositions relating to plants,seeds and derivatives of cucumber hybrid SV8975CB, cucumber line ISAACand cucumber BAP-TA-MATHYS-GY.

Cucumber hybrid SV8975CB shows resistance against CYSDV, CVYV, ZYMV,PRSV, WMV II, CMV, PM and scab. Cucumber hybrid SV8975CB hasarchitecture typical for summer with small leaves, short internodes, 2-3flowers per nodes, determined side shots, and very nice fruit quality.

A. Origin and Breeding History of Cucumber Hybrid SV8975CB

The parents of hybrid SV8975CB are ISAAC and BAP-TA-MATHYS-GY. Theseparents were created as follows:

BAP-TA-MATHYS-GY was created as follows:

-   -   a developmental cross was made between two gynoecious,        parthenocarpic lines carrying CYSDV resistance. Fruits of the        male were relatively shorter compared to the female and both        lines were elite.    -   the F1 was designated DRB6401 and self-pollinated in        Bergschenhoek, the Netherlands    -   an F2 individual, 06GT0873, was selected from 20-30 F2 plants        and selfed to the F3 generation in Bergschenhoek, the        Netherlands    -   an F3 plant, 07GT0191, was selected from 15-20 F3 individuals        and selfed to F4 in Bergschenhoek, the Netherlands    -   an F4 individual, 07GT0744, was selected from 12 F4 plants and        selfed to F5 in Bergschenhoek, the Netherlands    -   an F5 individual, 08GS0128, was selected from 7 F5 plants and        selfed to F6 in Bergschenhoek, the Netherlands    -   an F6 plant, 08GS0951, was selected from 7 F6 plants and        designated Mathys, a gynoecious, parthenocarpic line

CYSDV tests were not performed during the selfing and selectionprocesses, because both parents of the developmental cross were fixedfor CYSDV resistance. CYSDV resistance originated from two sources, oneof which was PI 250147 described in patent family containing EP 1 782685 A1.

ISAAC was created as follows:

-   -   a developmental cross was made between two gynoecious,        parthenocarpic lines carrying CYSDV resistance. Fruits of the        male were relatively shorter compared to the female and both        lines were elite.    -   the F1, 04GT0273, was self-pollinated in Bergschenhoek, the        Netherlands an F2 individual, 04GT0484, was selected from 20-30        F2 plants and selfed to the F3 generation in Bergschenhoek, the        Netherlands    -   an F3 plant, 06GT0177p, was selected from 15-20 F3 individuals        and selfed to F4 in Bergschenhoek, the Netherlands    -   an F4 individual, 06GT0790, was selected from 12 F4 plants and        selfed to F5 in Bergschenhoek, the Netherlands    -   an F5 individual, 06GT0790e, was selected from 7 F5 plants and        designated ISAAC, a gynoecious, parthenocarpic line

CYSDV tests were not performed during the selfing and selectionprocesses, because both parents of the developmental cross were fixedfor CYSDV resistance. CYSDV resistance originated from two sources, oneof which was PI 250147 described in patent family containing EP 1 782685 A1.

The parent lines are uniform and stable, as is a hybrid producedtherefrom. A small percentage of variants can occur within commerciallyacceptable limits for almost any characteristic during the course ofrepeated multiplication. However no variants are expected.

B. Physiological and Morphological Characteristics of Cucumber HybridSV8975CB, Cucumber Line ISAAC and Cucumber BAP-TA-MATHYS-GY

In accordance with one aspect of the present invention, there isprovided a plant having the physiological and morphologicalcharacteristics of cucumber hybrid SV8975CB and the parent linesthereof. A description of the physiological and morphologicalcharacteristics of such plants is presented in Tables 1-2.

TABLE 1 Physiological and Morphological Characteristics of HybridSV8975CB Characteristic SV8975CB 1. Type predominant usage slicing/freshmarket predominant culture greenhouse 2. Maturity days from seeding tomarket maturity 53 3. Plant habit vine cotyledon: bitterness absent(Rocket GS, Sandra) growth type indeterminate (Corona, Levina) time ofdevelopment of female medium flowers (80% of plants with at least onefemale flower) sex 100% gynoecious (plant species with all femaleflowers on the same plant) sex expression gynoecious When all the nodeshave only female flowers. Under certain conditions [darkness, cold,chemical treatment], a few male flowers will develop (Farbio, Sandra,Wilma) number of female flowers per node mostly 2 (Corona) flower coloryellow flower color (RHS color chart value) 9A 4. Main Stem main stemlength 218.73 cm number of nodes from cotyledon  1 leaves to nodebearing the first pistillate flower internode length 6.26 cm stem formgrooved, ridged plant: total length of first 15 short (Kora, Maran, Naf)internodes 5. Leaf attitude horizontal (Jazzer) mature blade of thirdleaf: leaf length 218.53 mm mature blade of third leaf: leaf width227.66 mm mature blade of third leaf: petiole 19.86 mm length lengthmedium (Briljant) ratio length of terminal lobe/length of small(Galileo) blade shape of apex of terminal lobe right-angled (Hana)intensity of green color light (De Russie) blistering absent or veryweak (Silor) undulation of margin moderate dentation of margin strong(Travito) ovary: color of vestiture white (Jazzer) 7. Fruit Setparthenocarpy present (Farbio, Rocket GS, Sandra, Wilma) length medium(Gemini, Jasser) 6. Fruit at edible maturity: fruit length 17.34 cmdiameter small (Picobello, Wilma) at edible maturity: fruit diameter at3.60 cm medial ratio length/diameter medium (Jazzer, Picobello, Wilma)core diameter in relation to diameter medium (Corona) of fruit shape intransverse section round to angular (Dasher) shape of stem end acute (DeMassy); obtuse (Maram, Score) shape of calyx end obtuse (Reno) at ediblematurity: fruit gram weight 166.73 gm skin color/mottling not mottled atedible maturity: yellowish blossom absent end stripes at ediblematurity: predominant color medium green at stem end at edible maturity:Predominant color N137A at stem end (RHS Color Chart value) at ediblematurity: predominant color medium green at blossom end at ediblematurity: predominant color 137A-N137A at blossom end (RHS Color Chartvalue) at edible maturity: fruit neck shape not necked at ediblematurity: fruit tapering stem end tapered at edible maturity: stem endcross circular section at edible maturity: medial cross circular;triangular section at edible maturity: blossom end cross triangularsection ground color of skin at market stage green (Corona) intensity ofground color of skin medium at edible maturity: skin thickness thin atedible maturity: skin ribs absent sutures absent (Corona, Hana) creasingpresent (Corona, Nabil) degree of creasing very weak (Silor) at ediblematurity: skin toughness tender at edible maturity: skin luster glossyat edible maturity: spine color white at edible maturity: spine qualityfine at edible maturity: spine density few type of vestiture hairs only(Silor) density of vestiture very sparse (Vert petit de Paris) wartsabsent (Diana) at edible maturity: flavor bitterfree length of stripesabsent or very short dots Absent (Sensation) glaucosity absent or veryweak (Corona) length of peduncle short (Admirable) ground color of skinat physiological green ripeness 7. Fruit seed at harvest maturitymeasurements fruit seed length parth measurements fruit seed diameter atparth medial color yellow color RHS Color Chart value N144A colorpattern not striped surface smooth netting slight or none 8. Seedsnumber of seeds per fruit parth *These are typical values. Values mayvary due to environment. Other values that are substantially equivalentare also within the scope of the invention.

TABLE 2 Physiological and Morphological Characteristics ofBAP-TA-MATHYS-GY Characteristic BAP-TA-MATHYS-GY 1. Type predominantusage slicing/fresh market predominant culture greenhouse 2. Maturitydays from seeding to market maturity 53 3. Plant habit vine cotyledon:bitterness absent (Rocket GS, Sandra) growth type indeterminate (Corona,Levina) time of development of female flowers medium (80% of plants withat least one female flower) sex 100% gynoecious (plant species with allfemale flowers on the same plant) sex expression gynoecious When all thenodes have only female flowers. Under certain conditions [darkness,cold, chemical treatment], a few male flowers will develop (Farbio,Sandra, Wilma) number of female flowers per node mostly 2 (Corona)flower color yellow flower color (RHS color chart value) 9A 4. Main Stemmain stem length 231.13 cm number of nodes from cotyledon leaves  1 tonode bearing the first pistillate flower internode length 6.18 cm stemform grooved, ridged plant: total length of first 15 internodes short(Kora, Maran, Naf) 5. Leaf attitude horizontal (Jazzer) mature blade ofthird leaf: leaf length 196.06 mm mature blade of third leaf: leaf width213.26 mm mature blade of third leaf: petiole 18.79 cm length lengthshort (Adam) ratio length of terminal lobe/length of large (Melody)blade shape of apex of terminal lobe obtuse (Melody) intensity of greencolor medium (Rocket GS, Stereo) blistering medium (Monir) undulation ofmargin strong (Tokyo Slicer) dentation of margin strong (Travito) ovary:color of vestiture white (Jazzer) 7. Fruit Set parthenocarpy present(Farbio, Rocket GS, Sandra, Wilma) length medium (Gemini, Jasser) 6.Fruit at edible maturity: fruit length 16.97 cm diameter medium (Corona,Diamant) at edible maturity: fruit diameter at 3.86 cm medial ratiolength/diameter medium (Jazzer, Picobello, Wilma) core diameter inrelation to diameter of medium (Corona) fruit shape in transversesection round (Telepathy, Susan) shape of stem end obtuse (Maram, Score)shape of calyx end obtuse (Reno) at edible maturity: fruit gram weight185 gm skin color/mottling not mottled at edible maturity: yellowishblossom absent end stripes at edible maturity: predominant color atmedium green stem end at edible maturity: Predominant color at N137Astem end (RHS Color Chart value) at edible maturity: predominant colorat medium green blossom end at edible maturity: predominant color at137A blossom end (RHS Color Chart value) at edible maturity: fruit neckshape not necked at edible maturity: fruit tapering blossom end taperedat edible maturity: stem end cross circular section at edible maturity:medial cross section circular at edible maturity: blossom end crosscircular; triangular section ground color of skin at market stage green(Corona) intensity of ground color of skin medium at edible maturity:skin thickness thin at edible maturity: skin ribs absent sutures absent(Corona, Hana) creasing present (Corona, Nabil) degree of creasing veryweak (Silor) at edible maturity: skin toughness tender at ediblematurity: skin luster glossy at edible maturity: spine color white atedible maturity: spine quality fine at edible maturity: spine densityfew type of vestiture hairs only (Silor) density of vestiture verysparse (Vert petit de Paris) warts absent (Diana) at edible maturity:flavor bitterfree length of stripes absent or very short dots absent(Sensation) glaucosity absent or very weak (Corona) length of pedunclemedium (Fendan) ground color of skin at physiological green ripeness 7.Fruit seed at harvest maturity measurements fruit seed length parthmeasurements fruit seed diameter at parth medial color yellow color RHSColor Chart value 145A color pattern not striped surface smooth nettingslight or none 8. Seeds number of seeds per fruit parth grams per 1,000seeds parth *These are typical values. Values may vary due toenvironment. Other values that are substantially equivalent are alsowithin the scope of the invention.

C. Breeding Cucumber Plants

One aspect of the current invention concerns methods for producing seedof cucumber hybrid SV8975CB involving crossing cucumber lines ISAAC andBAP-TA-MATHYS-GY. Alternatively, in other embodiments of the invention,hybrid SV8975CB, line ISAAC, or BAP-TA-MATHYS-GY may be crossed withitself or with any second plant. Such methods can be used forpropagation of hybrid SV8975CB and/or the cucumber lines ISAAC andBAP-TA-MATHYS-GY, or can be used to produce plants that are derived fromhybrid SV8975CB and/or the cucumber lines ISAAC and BAP-TA-MATHYS-GY.Plants derived from hybrid SV8975CB and/or the cucumber lines ISAAC andBAP-TA-MATHYS-GY may be used, in certain embodiments, for thedevelopment of new cucumber varieties.

The development of new varieties using one or more starting varieties iswell known in the art. In accordance with the invention, novel varietiesmay be created by crossing hybrid SV8975CB followed by multiplegenerations of breeding according to such well known methods. Newvarieties may be created by crossing with any second plant. In selectingsuch a second plant to cross for the purpose of developing novel lines,it may be desired to choose those plants which either themselves exhibitone or more selected desirable characteristics or which exhibit thedesired characteristic(s) when in hybrid combination. Once initialcrosses have been made, inbreeding and selection take place to producenew varieties. For development of a uniform line, often five or moregenerations of selfing and selection are involved.

Uniform lines of new varieties may also be developed by way ofdouble-haploids. This technique allows the creation of true breedinglines without the need for multiple generations of selfing andselection. In this manner true breeding lines can be produced in aslittle as one generation. Haploid embryos may be produced frommicrospores, pollen, anther cultures, or ovary cultures. The haploidembryos may then be doubled autonomously, or by chemical treatments(e.g. colchicine treatment). Alternatively, haploid embryos may be growninto haploid plants and treated to induce chromosome doubling. In eithercase, fertile homozygous plants are obtained. In accordance with theinvention, any of such techniques may be used in connection with a plantof the invention and progeny thereof to achieve a homozygous line.

Backcrossing can also be used to improve an inbred plant. Backcrossingtransfers a specific desirable trait from one inbred or non-inbredsource to an inbred that lacks that trait. This can be accomplished, forexample, by first crossing a superior inbred (A) (recurrent parent) to adonor inbred (non-recurrent parent), which carries the appropriate locusor loci for the trait in question. The progeny of this cross are thenmated back to the superior recurrent parent (A) followed by selection inthe resultant progeny for the desired trait to be transferred from thenon-recurrent parent. After five or more backcross generations withselection for the desired trait, the progeny have the characteristicbeing transferred, but are like the superior parent for most or almostall other loci. The last backcross generation would be selfed to givepure breeding progeny for the trait being transferred.

The plants of the present invention are particularly well suited for thedevelopment of new lines based on the elite nature of the geneticbackground of the plants. In selecting a second plant to cross withSV8975CB and/or cucumber lines ISAAC and BAP-TA-MATHYS-GY for thepurpose of developing novel cucumber lines, it will typically bepreferred to choose those plants which either themselves exhibit one ormore selected desirable characteristics or which exhibit the desiredcharacteristic(s) when in hybrid combination. Examples of desirabletraits may include, in specific embodiments, high seed yield, high seedgermination, seedling vigor, high fruit yield, disease tolerance orresistance, and adaptability for soil and climate conditions.Consumer-driven traits, such as a fruit shape, color, texture, and tasteare other examples of traits that may be incorporated into new lines ofcucumber plants developed by this invention.

D. Performance Characteristics

As described above, hybrid SV8975CB exhibits desirable traits, asconferred by cucumber lines ISAAC and BAP-TA-MATHYS-GY. The performancecharacteristics of hybrid SV8975CB and cucumber lines ISAAC andBAP-TA-MATHYS-GY were the subject of an objective analysis of theperformance traits relative to other varieties. The results of theanalysis are presented below.

TABLE 3 Performance Data of parent BAP-TA-MATHYS-GY FEM: ISAAC MALE:MATHYS CYSDV resistant resistant Cladosporium resistant resistantCorynespora susceptible susceptible PM resistant resistant CMV resistantresistant CVYV susceptible resistant ZYMV resistant resistant

E. Further Embodiments of the Invention

In certain aspects of the invention, plants described herein areprovided modified to include at least a first desired heritable trait.Such plants may, in one embodiment, be developed by a plant breedingtechnique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to a genetic locus transferred into the plant viathe backcrossing technique. The term single locus converted plant asused herein refers to those cucumber plants which are developed by aplant breeding technique called backcrossing, wherein essentially all ofthe morphological and physiological characteristics of a variety arerecovered in addition to the single locus transferred into the varietyvia the backcrossing technique. By essentially all of the morphologicaland physiological characteristics, it is meant that the characteristicsof a plant are recovered that are otherwise present when compared in thesame environment, other than an occasional variant trait that mightarise during backcrossing or direct introduction of a transgene.

Backcrossing methods can be used with the present invention to improveor introduce a characteristic into the present variety. The parentalcucumber plant which contributes the locus for the desiredcharacteristic is termed the nonrecurrent or donor parent. Thisterminology refers to the fact that the nonrecurrent parent is used onetime in the backcross protocol and therefore does not recur. Theparental cucumber plant to which the locus or loci from the nonrecurrentparent are transferred is known as the recurrent parent as it is usedfor several rounds in the backcrossing protocol.

In a typical backcross protocol, the original variety of interest(recurrent parent) is crossed to a second variety (nonrecurrent parent)that carries the single locus of interest to be transferred. Theresulting progeny from this cross are then crossed again to therecurrent parent and the process is repeated until a cucumber plant isobtained wherein essentially all of the morphological and physiologicalcharacteristics of the recurrent parent are recovered in the convertedplant, in addition to the single transferred locus from the nonrecurrentparent.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single trait or characteristic in the originalvariety. To accomplish this, a single locus of the recurrent variety ismodified or substituted with the desired locus from the nonrecurrentparent, while retaining essentially all of the rest of the desiredgenetic, and therefore the desired physiological and morphologicalconstitution of the original variety. The choice of the particularnonrecurrent parent will depend on the purpose of the backcross; one ofthe major purposes is to add some commercially desirable trait to theplant. The exact backcrossing protocol will depend on the characteristicor trait being altered and the genetic distance between the recurrentand nonrecurrent parents. Although backcrossing methods are simplifiedwhen the characteristic being transferred is a dominant allele, arecessive allele, or an additive allele (between recessive anddominant), may also be transferred. In this instance it may be necessaryto introduce a test of the progeny to determine if the desiredcharacteristic has been successfully transferred.

In one embodiment, progeny cucumber plants of a backcross in which aplant described herein is the recurrent parent comprise (i) the desiredtrait from the non-recurrent parent and (ii) all of the physiologicaland morphological characteristics of cucumber the recurrent parent asdetermined at the 5% significance level when grown in the sameenvironmental conditions.

New varieties can also be developed from more than two parents. Thetechnique, known as modified backcrossing, uses different recurrentparents during the backcrossing. Modified backcrossing may be used toreplace the original recurrent parent with a variety having certain moredesirable characteristics or multiple parents may be used to obtaindifferent desirable characteristics from each.

With the development of molecular markers associated with particulartraits, it is possible to add additional traits into an established germline, such as represented here, with the end result being substantiallythe same base germplasm with the addition of a new trait or traits.Molecular breeding, as described in Moose and Mumm, 2008 (PlantPhysiology, 147: 969-977), for example, and elsewhere, provides amechanism for integrating single or multiple traits or QTL into an eliteline. This molecular breeding-facilitated movement of a trait or traitsinto an elite line may encompass incorporation of a particular genomicfragment associated with a particular trait of interest into the eliteline by the mechanism of identification of the integrated genomicfragment with the use of flanking or associated marker assays. In theembodiment represented here, one, two, three or four genomic loci, forexample, may be integrated into an elite line via this methodology. Whenthis elite line containing the additional loci is further crossed withanother parental elite line to produce hybrid offspring, it is possibleto then incorporate at least eight separate additional loci into thehybrid. These additional loci may confer, for example, such traits as adisease resistance or a fruit quality trait. In one embodiment, eachlocus may confer a separate trait. In another embodiment, loci may needto be homozygous and exist in each parent line to confer a trait in thehybrid. In yet another embodiment, multiple loci may be combined toconfer a single robust phenotype of a desired trait.

Many single locus traits have been identified that are not regularlyselected for in the development of a new inbred but that can be improvedby backcrossing techniques. Single locus traits may or may not betransgenic; examples of these traits include, but are not limited to,herbicide resistance, resistance to bacterial, fungal, or viral disease,insect resistance, modified fatty acid or carbohydrate metabolism, andaltered nutritional quality. These comprise genes generally inheritedthrough the nucleus.

Direct selection may be applied where the single locus acts as adominant trait. For this selection process, the progeny of the initialcross are assayed for viral resistance and/or the presence of thecorresponding gene prior to the backcrossing. Selection eliminates anyplants that do not have the desired gene and resistance trait, and onlythose plants that have the trait are used in the subsequent backcross.This process is then repeated for all additional backcross generations.

Selection of cucumber plants for breeding is not necessarily dependenton the phenotype of a plant and instead can be based on geneticinvestigations. For example, one can utilize a suitable genetic markerwhich is closely genetically linked to a trait of interest. One of thesemarkers can be used to identify the presence or absence of a trait inthe offspring of a particular cross, and can be used in selection ofprogeny for continued breeding. This technique is commonly referred toas marker assisted selection. Any other type of genetic marker or otherassay which is able to identify the relative presence or absence of atrait of interest in a plant can also be useful for breeding purposes.Procedures for marker assisted selection are well known in the art. Suchmethods will be of particular utility in the case of recessive traitsand variable phenotypes, or where conventional assays may be moreexpensive, time consuming or otherwise disadvantageous. Types of geneticmarkers which could be used in accordance with the invention include,but are not necessarily limited to, Simple Sequence Length Polymorphisms(SSLPs) (Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990),Randomly Amplified Polymorphic DNAs (RAPDs), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Arbitrary Primed Polymerase Chain Reaction (AP-PCR), Amplified FragmentLength Polymorphisms (AFLPs) (EP 534 858, specifically incorporatedherein by reference in its entirety), and Single NucleotidePolymorphisms (SNPs) (Wang et al., Science, 280:1077-1082, 1998).

F. Plants Derived by Genetic Engineering

Many useful traits that can be introduced by backcrossing, as well asdirectly into a plant, are those which are introduced by genetictransformation techniques. Genetic transformation may therefore be usedto insert a selected transgene into a plant of the invention or may,alternatively, be used for the preparation of transgenes which can beintroduced by backcrossing. Methods for the transformation of plantsthat are well known to those of skill in the art and applicable to manycrop species include, but are not limited to, electroporation,microprojectile bombardment, Agrobacterium-mediated transformation anddirect DNA uptake by protoplasts.

To effect transformation by electroporation, one may employ eitherfriable tissues, such as a suspension culture of cells or embryogeniccallus or alternatively one may transform immature embryos or otherorganized tissue directly. In this technique, one would partiallydegrade the cell walls of the chosen cells by exposing them topectin-degrading enzymes (pectolyases) or mechanically wound tissues ina controlled manner.

An efficient method for delivering transforming DNA segments to plantcells is microprojectile bombardment. In this method, particles arecoated with nucleic acids and delivered into cells by a propellingforce. Exemplary particles include those comprised of tungsten,platinum, and preferably, gold. For the bombardment, cells in suspensionare concentrated on filters or solid culture medium. Alternatively,immature embryos or other target cells may be arranged on solid culturemedium. The cells to be bombarded are positioned at an appropriatedistance below the macroprojectile stopping plate.

An illustrative embodiment of a method for delivering DNA into plantcells by acceleration is the Biolistics Particle Delivery System, whichcan be used to propel particles coated with DNA or cells through ascreen, such as a stainless steel or Nytex screen, onto a surfacecovered with target cells. The screen disperses the particles so thatthey are not delivered to the recipient cells in large aggregates.Microprojectile bombardment techniques are widely applicable, and may beused to transform virtually any plant species.

Agrobacterium-mediated transfer is another widely applicable system forintroducing gene loci into plant cells. An advantage of the technique isthat DNA can be introduced into whole plant tissues, thereby bypassingthe need for regeneration of an intact plant from a protoplast. ModernAgrobacterium transformation vectors are capable of replication in E.coli as well as Agrobacterium, allowing for convenient manipulations(Klee et al., Bio-Technology, 3(7):637-642, 1985). Moreover, recenttechnological advances in vectors for Agrobacterium-mediated genetransfer have improved the arrangement of genes and restriction sites inthe vectors to facilitate the construction of vectors capable ofexpressing various polypeptide coding genes. The vectors described haveconvenient multi-linker regions flanked by a promoter and apolyadenylation site for direct expression of inserted polypeptidecoding genes. Additionally, Agrobacterium containing both armed anddisarmed Ti genes can be used for transformation.

In those plant strains where Agrobacterium-mediated transformation isefficient, it is the method of choice because of the facile and definednature of the gene locus transfer. The use of Agrobacterium-mediatedplant integrating vectors to introduce DNA into plant cells is wellknown in the art (Fraley et al., Bio/Technology, 3:629-635, 1985; U.S.Pat. No. 5,563,055).

Transformation of plant protoplasts also can be achieved using methodsbased on calcium phosphate precipitation, polyethylene glycol treatment,electroporation, and combinations of these treatments (see, e.g.,Potrykus et al., Mol. Gen. Genet., 199:183-188, 1985; Omirulleh et al.,Plant Mol. Biol., 21(3):415-428, 1993; Fromm et al., Nature,312:791-793, 1986; Uchimiya et al., Mol. Gen. Genet., 204:204, 1986;Marcotte et al., Nature, 335:454, 1988). Transformation of plants andexpression of foreign genetic elements is exemplified in Choi et al.(Plant Cell Rep., 13: 344-348, 1994), and Ellul et al. (Theor. Appl.Genet., 107:462-469, 2003).

A number of promoters have utility for plant gene expression for anygene of interest including but not limited to selectable markers,scoreable markers, genes for pest tolerance, disease resistance,nutritional enhancements and any other gene of agronomic interest.Examples of constitutive promoters useful for plant gene expressioninclude, but are not limited to, the cauliflower mosaic virus (CaMV)P-35S promoter, which confers constitutive, high-level expression inmost plant tissues (see, e.g., Odel et al., Nature, 313:810, 1985),including in monocots (see, e.g., Dekeyser et al., Plant Cell, 2:591,1990; Terada and Shimamoto, Mol. Gen. Genet., 220:389, 1990); a tandemlyduplicated version of the CaMV 35S promoter, the enhanced 35S promoter(P-e35S); 1 the nopaline synthase promoter (An et al., Plant Physiol.,88:547, 1988); the octopine synthase promoter (Fromm et al., Plant Cell,1:977, 1989); and the figwort mosaic virus (P-FMV) promoter as describedin U.S. Pat. No. 5,378,619 and an enhanced version of the FMV promoter(P-eFMV) where the promoter sequence of P-FMV is duplicated in tandem;the cauliflower mosaic virus 19S promoter; a sugarcane bacilliform viruspromoter; a commelina yellow mottle virus promoter; and other plant DNAvirus promoters known to express in plant cells.

A variety of plant gene promoters that are regulated in response toenvironmental, hormonal, chemical, and/or developmental signals can alsobe used for expression of an operably linked gene in plant cells,including promoters regulated by (1) heat (Callis et al., PlantPhysiol., 88:965, 1988), (2) light (e.g., pea rbcS-3A promoter,Kuhlemeier et al., Plant Cell, 1:471, 1989; maize rbcS promoter,Schaffner and Sheen, Plant Cell, 3:997, 1991; or chlorophyll a/b-bindingprotein promoter, Simpson et al., EMBO J., 4:2723, 1985), (3) hormones,such as abscisic acid (Marcotte et al., Plant Cell, 1:969, 1989), (4)wounding (e.g., wunl, Siebertz et al., Plant Cell, 1:961, 1989); or (5)chemicals such as methyl jasmonate, salicylic acid, or Safener. It mayalso be advantageous to employ organ-specific promoters (e.g., Roshal etal., EMBO J., 6:1155, 1987; Schernthaner et al., EMBO J., 7:1249, 1988;Bustos et al., Plant Cell, 1:839, 1989).

Exemplary nucleic acids which may be introduced to plants of thisinvention include, for example, DNA sequences or genes from anotherspecies, or even genes or sequences which originate with or are presentin the same species, but are incorporated into recipient cells bygenetic engineering methods rather than classical reproduction orbreeding techniques. However, the term “exogenous” is also intended torefer to genes that are not normally present in the cell beingtransformed, or perhaps simply not present in the form, structure, etc.,as found in the transforming DNA segment or gene, or genes which arenormally present and that one desires to express in a manner thatdiffers from the natural expression pattern, e.g., to over-express.Thus, the term “exogenous” gene or DNA is intended to refer to any geneor DNA segment that is introduced into a recipient cell, regardless ofwhether a similar gene may already be present in such a cell. The typeof DNA included in the exogenous DNA can include DNA which is alreadypresent in the plant cell, DNA from another plant, DNA from a differentorganism, or a DNA generated externally, such as a DNA sequencecontaining an antisense message of a gene, or a DNA sequence encoding asynthetic or modified version of a gene.

Many hundreds if not thousands of different genes are known and couldpotentially be introduced into a cucumber plant according to theinvention. Non-limiting examples of particular genes and correspondingphenotypes one may choose to introduce into a cucumber plant include oneor more genes for insect tolerance, such as a Bacillus thuringiensis(B.t.) gene, pest tolerance such as genes for fungal disease control,herbicide tolerance such as genes conferring glyphosate tolerance, andgenes for quality improvements such as yield, nutritional enhancements,environmental or stress tolerances, or any desirable changes in plantphysiology, growth, development, morphology or plant product(s). Forexample, structural genes would include any gene that confers insecttolerance including but not limited to a Bacillus insect control proteingene as described in WO 99/31248, herein incorporated by reference inits entirety, U.S. Pat. No. 5,689,052, herein incorporated by referencein its entirety, U.S. Pat. Nos. 5,500,365 and 5,880,275, hereinincorporated by reference in their entirety. In another embodiment, thestructural gene can confer tolerance to the herbicide glyphosate asconferred by genes including, but not limited to Agrobacterium strainCP4 glyphosate resistant EPSPS gene (aroA:CP4) as described in U.S. Pat.No. 5,633,435, herein incorporated by reference in its entirety, orglyphosate oxidoreductase gene (GOX) as described in U.S. Pat. No.5,463,175, herein incorporated by reference in its entirety.

Alternatively, the DNA coding sequences can affect these phenotypes byencoding a non-translatable RNA molecule that causes the targetedinhibition of expression of an endogenous gene, for example viaantisense- or cosuppression-mediated mechanisms (see, for example, Birdet al., Biotech. Gen. Engin. Rev., 9:207, 1991). The RNA could also be acatalytic RNA molecule (i.e., a ribozyme) engineered to cleave a desiredendogenous mRNA product (see for example, Gibson and Shillito, Mol.Biotech., 7:125, 1997). Thus, any gene which produces a protein or mRNAwhich expresses a phenotype or morphology change of interest is usefulfor the practice of the present invention.

G. Definitions

In the description and tables herein, a number of terms are used. Inorder to provide a clear and consistent understanding of thespecification and claims, the following definitions are provided:

Allele: Any of one or more alternative forms of a gene locus, all ofwhich alleles relate to one trait or characteristic. In a diploid cellor organism, the two alleles of a given gene occupy corresponding locion a pair of homologous chromosomes.

Backcrossing: A process in which a breeder repeatedly crosses hybridprogeny, for example a first generation hybrid (F₁), back to one of theparents of the hybrid progeny. Backcrossing can be used to introduce oneor more single locus conversions from one genetic background intoanother.

Crossing: The mating of two parent plants.

Cross-pollination: Fertilization by the union of two gametes fromdifferent plants.

Diploid: A cell or organism having two sets of chromosomes.

Emasculate: The removal of plant male sex organs or the inactivation ofthe organs with a cytoplasmic or nuclear genetic factor or a chemicalagent conferring male sterility.

Enzymes: Molecules which can act as catalysts in biological reactions.

F₁ Hybrid: The first generation progeny of the cross of two nonisogenicplants.

Genotype: The genetic constitution of a cell or organism.

Haploid: A cell or organism having one set of the two sets ofchromosomes in a diploid.

Linkage: A phenomenon wherein alleles on the same chromosome tend tosegregate together more often than expected by chance if theirtransmission was independent.

Marker: A readily detectable phenotype, preferably inherited incodominant fashion (both alleles at a locus in a diploid heterozygoteare readily detectable), with no environmental variance component, i.e.,heritability of 1.

Phenotype: The detectable characteristics of a cell or organism, whichcharacteristics are the manifestation of gene expression.

Quantitative Trait Loci (QTL): Quantitative trait loci (QTL) refer togenetic loci that control to some degree numerically representabletraits that are usually continuously distributed.

Resistance: As used herein, the terms “resistance” and “tolerance” areused interchangeably to describe plants that show no symptoms to aspecified biotic pest, pathogen, abiotic influence or environmentalcondition. These terms are also used to describe plants showing somesymptoms but that are still able to produce marketable product with anacceptable yield. Some plants that are referred to as resistant ortolerant are only so in the sense that they may still produce a crop,even though the plants are stunted and the yield is reduced.

Regeneration: The development of a plant from tissue culture.

Royal Horticultural Society (RHS) color chart value: The RHS color chartis a standardized reference which allows accurate identification of anycolor. A color's designation on the chart describes its hue, brightnessand saturation. A color is precisely named by the RHS color chart byidentifying the group name, sheet number and letter, e.g., Yellow-OrangeGroup 19A or Red Group 41B.

Self-pollination: The transfer of pollen from the anther to the stigmaof the same plant.

Single Locus Converted (Conversion) Plant: Plants which are developed bya plant breeding technique called backcrossing, wherein essentially allof the morphological and physiological characteristics of a cucumbervariety are recovered in addition to the characteristics of the singlelocus transferred into the variety via the backcrossing technique and/orby genetic transformation.

Substantially Equivalent: A characteristic that, when compared, does notshow a statistically significant difference (e.g., p=0.05) from themean.

Tissue Culture: A composition comprising isolated cells of the same or adifferent type or a collection of such cells organized into parts of aplant.

Transgene: A genetic locus comprising a sequence which has beenintroduced into the genome of a cucumber plant by transformation.

H. Deposit Information

A deposit of cucumber hybrid SV8975CB and inbred parent lines ISAAC andBAP-TA-MATHYS-GY, disclosed above and recited in the claims, has beenmade with the American Type Culture Collection (ATCC), 10801 UniversityBlvd., Manassas, Va. 20110-2209. The date of deposit was Jul. 12, 2013.The accession numbers for those deposited seeds of cucumber hybridSV8975CB and inbred parent line BAP-TA-MATHYS-GY are ATCC Accession No.PTA-120466, and ATCC Accession No. PTA-120467, respectively. Uponissuance of a patent, all restrictions upon the deposits will beremoved, and the deposits are intended to meet all of the requirementsof 37 C.F.R. §1.801-1.809. The deposits will be maintained in thedepository for a period of 30 years, or 5 years after the last request,or for the effective life of the patent, whichever is longer, and willbe replaced if necessary during that period.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity andunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the invention, as limited only bythe scope of the appended claims.

All references cited herein are hereby expressly incorporated herein byreference.

What is claimed is:
 1. A cucumber plant comprising at least a first set of the chromosomes of cucumber line BAP-TA-MATHYS-GY, a sample of seed of said line having been deposited under ATCC Accession Number PTA-120467.
 2. A seed comprising at least a first set of the chromosomes of cucumber line BAP-TA-MATHYS-GY, a sample of seed of said line having been deposited under ATCC Accession Number PTA-120467.
 3. The plant of claim 1, which is an inbred.
 4. The plant of claim 1, which is a hybrid.
 5. The seed of claim 2, which is an inbred.
 6. The seed of claim 2, which is a hybrid.
 7. The plant of claim 4, wherein the hybrid plant is cucumber hybrid SV8975CB, a sample of seed of said hybrid SV8975CB having been deposited under ATCC Accession Number PTA-120466.
 8. The seed of claim 6, defined as a seed of cucumber hybrid SV8975CB, a sample of seed of said hybrid SV8975CB having been deposited under ATCC Accession Number PTA-120466.
 9. The seed of claim 2, defined as a seed of line BAP-TA-MATHYS-GY.
 10. A plant part of the plant of claim
 1. 11. The plant part of claim 10, further defined as a leaf, an ovule, pollen, a fruit, or a cell.
 12. A cucumber plant having all the physiological and morphological characteristics of the cucumber plant of claim
 7. 13. A tissue culture of regenerable cells of the plant of claim
 1. 14. The tissue culture according to claim 13, comprising cells or protoplasts from a plant part selected from the group consisting of embryos, meristems, cotyledons, pollen, leaves, anthers, roots, root tips, pistil, flower, seed and stalks.
 15. A cucumber plant regenerated from the tissue culture of claim
 13. 16. A method of vegetatively propagating the cucumber plant of claim 1 comprising the steps of: (a) collecting tissue capable of being propagated from the plant according to claim 1; (b) cultivating said tissue to obtain proliferated shoots; and (c) rooting said proliferated shoots to obtain rooted plantlets.
 17. The method of claim 16, further comprising growing at least a first cucumber plant from said rooted plantlets.
 18. A method of introducing a desired trait into a cucumber line comprising: (a) crossing a plant of line BAP-TA-MATHYS-GY with a second cucumber plant that comprises a desired trait to produce F1 progeny, a sample of seed of said line having been deposited under ATCC Accession Number PTA-120467; (b) selecting an F1 progeny that comprises the desired trait; (c) backcrossing the selected F1 progeny with a plant of line BAP-TA-MATHYS-GY to produce backcross progeny; (d) selecting backcross progeny comprising the desired trait and the physiological and morphological characteristic of cucumber line BAP-TA-MATHYS-GY; and (e) repeating steps (c) and (d) three or more times to produce selected fourth or higher backcross progeny that comprise the desired trait.
 19. A cucumber plant produced by the method of claim
 18. 20. A method of producing a cucumber plant comprising an added trait, the method comprising introducing a transgene conferring the trait into a plant of cucumber hybrid SV8975CB, or cucumber line BAP-TA-MATHYS-GY, a sample of seed of said hybrid and line having been deposited under ATCC Accession Number PTA-120466, and ATCC Accession Number PTA-120467, respectively.
 21. A plant produced by the method of claim
 20. 22. The plant of claim 1, further comprising a transgene.
 23. The plant of claim 22, wherein the transgene confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, modified fatty acid metabolism, environmental stress tolerance, modified carbohydrate metabolism and modified protein metabolism.
 24. The plant of claim 1, further comprising a single locus conversion.
 25. The plant of claim 24, wherein the single locus conversion confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, modified fatty acid metabolism, environmental stress tolerance, modified carbohydrate metabolism and modified protein metabolism.
 26. A method for producing a seed of a cucumber plant derived from at least one of cucumber hybrid SV8975CB, or cucumber line BAP-TA-MATHYS-GY comprising the steps of: (a) crossing a cucumber plant of hybrid SV8975CB, or line BAP-TA-MATHYS-GY with itself or a second cucumber plant; a sample of seed of said hybrid and line having been deposited under ATCC Accession Number PTA-120466, and ATCC Accession Number PTA-120467, respectively; and (b) allowing seed of a hybrid SV8975CB, or line BAP-TA-MATHYS-GY-derived cucumber plant to form.
 27. The method of claim 26, further comprising the steps of: (c) selfing a plant grown from said hybrid SV8975CB, or BAP-TA-MATHYS-GY-derived cucumber seed to yield additional hybrid SV8975CB, or line BAP-TA-MATHYS-GY-derived cucumber seed; (d) growing said additional hybrid SV8975CB, or line BAP-TA-MATHYS-GY-derived cucumber seed of step (c) to yield additional hybrid SV8975CB, or line BAP-TA-MATHYS-GY-derived cucumber plants; and (e) repeating the crossing and growing steps of (c) and (d) to generate at least a first further hybrid SV8975CB, or line BAP-TA-MATHYS-GY-derived cucumber plant.
 28. The method of claim 26, wherein the second cucumber plant is of an inbred cucumber line.
 29. The method of claim 27, further comprising: (f) crossing the further hybrid SV8975CB, or BAP-TA-MATHYS-GY-derived cucumber plant with a second cucumber plant to produce seed of a hybrid progeny plant.
 30. A plant part of the plant of claim
 7. 31. The plant part of claim 30, further defined as a leaf, an ovule, pollen, a fruit, or a cell.
 32. A method of producing a cucumber seed comprising crossing the plant of claim 1 with itself or a second cucumber plant and allowing seed to form.
 33. A method of producing a cucumber comprising: (a) obtaining the plant according to claim 1, wherein the plant has been cultivated to maturity; and (b) collecting a cucumber from the plant. 